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Frontiers in Chemistry 2021In this mini-review, we briefly describe certain recently developed applications of the surface-enhanced Raman spectroscopy (SERS) for determining various biochemically... (Review)
Review
In this mini-review, we briefly describe certain recently developed applications of the surface-enhanced Raman spectroscopy (SERS) for determining various biochemically (especially medically) important species from ones as simple as hydrogen cations to those as complex as specific DNA fragments. We present a SERS analysis of species whose characterization is important to our understanding of various mechanisms in the human body and to show its potential as an alternative for methods routinely used in diagnostics and clinics. Furthermore, we explain how such SERS-based sensors operate and point out future prospects in this field.
PubMed: 34026727
DOI: 10.3389/fchem.2021.664134 -
Biosensors Feb 2022The accumulation of metal ions in the body is caused by human activities and industrial uses. Among these metal ions, copper is the third most abundant ion found in the...
The accumulation of metal ions in the body is caused by human activities and industrial uses. Among these metal ions, copper is the third most abundant ion found in the human body and is indispensable for health because it works as a catalyst in the iron absorption processes. However, high doses of copper ions have been reported to generate various diseases. Different types of sensors are used to detect metal ions for several applications. To design selective and specific recognition sites on the sensor surfaces, molecular imprinting is one of the most used alteration methods to detect targets by mimicking natural recognition molecules. In this study, an ion-imprinted polymer-integrated plasmonic sensor was prepared to selectively detect copper (Cu(II)) ions in real-time. Following different characterization experiments, the Cu(II)-imprinted plasmonic sensor was employed for kinetic, selectivity, and reusability studies. According to the results, it was observed that this sensor can measure with 96% accuracy in the Cu(II) concentration range of 0.04-5 μM in buffer solution. The limit of detection and limit of quantification values were computed as 0.027 µM and 0.089 µM. The results also showed that this plasmonic sensor works successfully not only in a buffer solution but also in complex media such as plasma and urine.
Topics: Copper; Humans; Ions; Metals; Molecular Imprinting; Polymers
PubMed: 35200351
DOI: 10.3390/bios12020091 -
PloS One 2022Body segment parameters are inputs for a range of applications. Participant-specific estimates of body segment parameters are desirable as this requires fewer prior...
Body segment parameters are inputs for a range of applications. Participant-specific estimates of body segment parameters are desirable as this requires fewer prior assumptions and can reduce outcome measurement errors. Commonly used methods for estimating participant-specific body segment parameters are either expensive and out of reach (medical imaging), have many underlying assumptions (geometrical modelling) or are based on a specific subset of a population (regression models). Our objective was to develop a participant-specific 3D scanning and body segmentation method that estimates body segment parameters without any assumptions about the geometry of the body, ethnic background, and gender, is low-cost, fast, and can be readily available. Using a Microsoft Kinect Version 2 camera, we developed a 3D surface scanning protocol that enabled the estimation of participant-specific body segment parameters. To evaluate our system, we performed repeated 3D scans of 21 healthy participants (10 male, 11 female). We used open source tools to segment each body scan into 16 segments (head, torso, abdomen, pelvis, left and right hand, forearm, upper arm, foot, shank and thigh) and wrote custom software to estimate each segment's mass, mass moment of inertia in the three principal orthogonal axes relevant to the center of the segment, longitudinal length, and center of mass. We compared our body segment parameter estimates to those obtained using two comparison methods and found that our system was consistent in estimating total body volume between repeated scans (male p = 0.1194, female p = 0.2240), estimated total body mass without significant differences when compared to our comparison method and a medical scale (male p = 0.8529, female p = 0.6339), and generated consistent and comparable estimates across a range of the body segment parameters of interest. Our work here outlines and provides the code for an inexpensive 3D surface scanning method for estimating a range of participant-specific body segment parameters.
Topics: Adult; Body Composition; Female; Human Body; Humans; Imaging, Three-Dimensional; Male; Software; Young Adult
PubMed: 34986175
DOI: 10.1371/journal.pone.0262296 -
International Journal of Thermal... Sep 2022Several studies on vaccines and medicines against virus-based illnesses (COVID-19, SARS, MERS) are being conducted worldwide. However, virus mutation is an issue.... (Review)
Review
Several studies on vaccines and medicines against virus-based illnesses (COVID-19, SARS, MERS) are being conducted worldwide. However, virus mutation is an issue. Therefore, inactivation and disinfection of viruses are crucial. This paper presents a method for virus inactivation by physical techniques. The infrared (IR) technique is preferred over other disinfection techniques such as ultraviolet (UV) and chemical disinfectants (alcohol) due to the associated health and environmental benefits. In this study, IR sources with various wavelengths were characterized and a far infrared (FIR) source was used to inactivate viruses. FIR sources have a therapeutic effect on the human body and have been used in medical centers. Virus spread is highly affected by environmental conditions such as temperature, humidity, and airflow. A setup with IR sources, an IR camera, an automatically controlled humidity chamber, and an airflow unit was constructed to study the viability of viruses in stationary droplets as a function of relative humidity and temperature. Bacteriophage was used as a model organism for studying enveloped viruses such as influenza and coronavirus. IR techniques were used for studying virus inactivation. The effect of various physical conditions such as temperature, humidity, and airflows was considered to study the effect of radiation on the stationary droplets of . All measurements were performed under laboratory conditions with controlled temperature and humidity. The IR camera system was used to measure the surface temperature of Phi6 suspension droplets. The samples subjected to IR radiation were processed for plaque assay preparation and counting. Measurements were carried out to reduce and eliminate droplets, which are one of the transmission pathways of viruses. IR was radiated in closed and open-air conditions with appropriate humidity and temperature. This study reports the effective inactivation of viruses by FIR. The inactivation rate under 50 %rh for IR radiated at 1.4 m height for 3 h in closed environmental chamber was 90%, and that under an airflow rate of 0.20 m/s for 10 min in open-air conditions at a height of 1.0 m was 45.7%.
PubMed: 35692600
DOI: 10.1016/j.ijthermalsci.2022.107595 -
Blood Purification 2017Over 50% of the human body is comprised of fluids that are distributed in defined compartments. Although compartmentalized, these fluids are dynamically connected.... (Review)
Review
Over 50% of the human body is comprised of fluids that are distributed in defined compartments. Although compartmentalized, these fluids are dynamically connected. Fluids, electrolytes, and acid-base balance in each compartment are tightly regulated, mostly in an energy-dependent manner to achieve their designed functions. For over a century, our understanding of the microvascular fluid homeostasis has evolved from hypothesized Ernest Starling principle to evidence-based and the revised Starling principle, incorporating the functional endothelial surface layer. The kidney is a highly vascular and encapsulated organ that is exquisitely sensitive to inadequate (insufficient or excess) blood flow. The kidney is particularly sensitive to venous congestion, and studies show that reduced venous return triggers a greater degree of kidney damage than that from lacking arterial flow. Thus, fluid overload can induce severe and sustained kidney injury. In the setting of established acute kidney injury, fluid management can be challenging. Impaired capacity of urine output and urine concentration and dilution should be taken into consideration when designing fluid therapy. Video Journal Club 'Cappuccino with Claudio Ronco' at http://www.karger.com/?doi=452702.
Topics: Acute Kidney Injury; Body Fluids; Fluid Therapy; Homeostasis; Humans
PubMed: 28114128
DOI: 10.1159/000452702 -
Frontiers in Microbiology 2021Recent advances in 3D printing have led to a rise in the use of 3D printed materials in prosthetics and external medical devices. These devices, while inexpensive, have...
Recent advances in 3D printing have led to a rise in the use of 3D printed materials in prosthetics and external medical devices. These devices, while inexpensive, have not been adequately studied for their ability to resist biofouling and biofilm buildup. Bacterial biofilms are a major cause of biofouling in the medical field and, therefore, hospital-acquired, and medical device infections. These surface-attached bacteria are highly recalcitrant to conventional antimicrobial agents and result in chronic infections. During the COVID-19 pandemic, the U.S. Food and Drug Administration and medical officials have considered 3D printed medical devices as alternatives to conventional devices, due to manufacturing shortages. This abundant use of 3D printed devices in the medical fields warrants studies to assess the ability of different microorganisms to attach and colonize to such surfaces. In this study, we describe methods to determine bacterial biofouling and biofilm formation on 3D printed materials. We explored the biofilm-forming ability of multiple opportunistic pathogens commonly found on the human body including , , and to colonize eight commonly used polylactic acid (PLA) polymers. Biofilm quantification, surface topography, digital optical microscopy, and 3D projections were employed to better understand the bacterial attachment to 3D printed surfaces. We found that biofilm formation depends on surface structure, hydrophobicity, and that there was a wide range of antimicrobial properties among the tested polymers. We compared our tested materials with commercially available antimicrobial PLA polymers.
PubMed: 34122361
DOI: 10.3389/fmicb.2021.646303 -
Materials Today. Bio Jan 2022Metabolites are important biomarkers in human body fluids, conveying direct information of cellular activities and physical conditions. Metabolite detection has long...
Metabolites are important biomarkers in human body fluids, conveying direct information of cellular activities and physical conditions. Metabolite detection has long been a research hotspot in the field of biology and medicine. Surface-enhanced Raman spectroscopy (SERS), based on the molecular "fingerprint" of Raman spectrum and the enormous signal enhancement (down to a single-molecule level) by plasmonic nanomaterials, has proven to be a novel and powerful tool for metabolite detection. SERS provides favorable properties such as ultra-sensitive, label-free, rapid, specific, and non-destructive detection processes. In this review, we summarized the progress in recent 10 years on SERS-based sensing of endogenous metabolites at the cellular level, in tissues, and in biofluids, as well as drug metabolites in biofluids. We made detailed discussions on the challenges and optimization methods of SERS technique in metabolite detection. The combination of SERS with modern biomedical technology were also anticipated.
PubMed: 35118368
DOI: 10.1016/j.mtbio.2022.100205 -
Journal of Cell Science Mar 2020Membrane mucins cover most mucosal surfaces throughout the human body. The intestine harbors complex population of microorganisms (the microbiota) and numerous exogenous... (Review)
Review
Membrane mucins cover most mucosal surfaces throughout the human body. The intestine harbors complex population of microorganisms (the microbiota) and numerous exogenous molecules that can harm the epithelium. In the colon, where the microbial burden is high, a mucus barrier forms the first line of defense by keeping bacteria away from the epithelial cells. In the small intestine where the mucus layer is less organized, microbes are kept at bay by peristalsis and antimicrobial peptides. Additionally, a dense glycocalyx consisting of extended and heavily glycosylated membrane mucins covers the surface of enterocytes. Whereas many aspects of mucosal barriers are being discovered, the function of membrane mucins remains a largely overlooked topic, mainly because we lack the necessary reagents and experimental animal models to investigate these large glycoproteins. In this Cell Science at a Glance article and accompanying poster, we highlight central concepts of membrane mucin biology and the role of membrane mucins as integral components of intestinal mucosal barriers. We also present the current consensus concerning the role of membrane mucins in host-microbe interactions. Moreover, we discuss how regulatory circuits that govern membrane mucins in the healthy gut display strong overlap with pathways that are perturbed during chronic inflammation. Finally, we review how dysregulation of intestinal membrane mucins may contribute to human diseases, such as inflammation and cancer.
Topics: Animals; Enterocytes; Epithelial Cells; Host Microbial Interactions; Humans; Intestinal Mucosa; Mucins
PubMed: 32169835
DOI: 10.1242/jcs.240929 -
Biosensors Nov 2022Heavy metal ions (HMIs) pose a serious threat to the environment and human body because they are toxic and non-biodegradable and widely exist in environmental... (Review)
Review
Heavy metal ions (HMIs) pose a serious threat to the environment and human body because they are toxic and non-biodegradable and widely exist in environmental ecosystems. It is necessary to develop a rapid, sensitive and convenient method for HMIs detection to provide a strong guarantee for ecology and human health. Ion-imprinted electrochemical sensors (IIECSs) based on nanomaterials have been regarded as an excellent technology because of the good selectivity, the advantages of fast detection speed, low cost, and portability. Electrode surfaces modified with nanomaterials can obtain excellent nano-effects, such as size effect, macroscopic quantum tunneling effect and surface effect, which greatly improve its surface area and conductivity, so as to improve the detection sensitivity and reduce the detection limit of the sensor. Hence, the present review focused on the fundamentals and the synthetic strategies of ion-imprinted polymers (IIPs) and IIECSs for HMIs detection, as well as the applications of various nanomaterials as modifiers and sensitizers in the construction of HMIIECSs and the influence on the sensing performance of the fabricated sensors. Finally, the potential challenges and outlook on the future development of the HMIIECSs technology were also highlighted. By means of the points presented in this review, we hope to provide some help in further developing the preparation methods of high-performance HMIIECSs and expanding their potential applications.
Topics: Humans; Ecosystem; Metals, Heavy; Nanostructures; Polymers; Ions
PubMed: 36551065
DOI: 10.3390/bios12121096